Campus Facilities
All life sciences research laboratories occupy contiguous space with in university's science complex. The Department of Biology is well equipped with research facilities.
Lokey Labs Electron Microscopy Facility
The Electron Microscopy Facility is well equipped for ultrastructural and biomaterials research. Used by a wide range of life science and materials researchers at University of Oregon the facility comprises of an FEI Titan 300kV analytical TEM/STEM, a Zeiss Ultra 55 SEM, and an FEI Helios DB-FIB with cryo capabilities. The lab also houses ultramicrotomes, evaporators, freeze-fracture, and other sample preparation equipment. A 120kV TEM with cryo capabilities will be added soon.
For prospective students unfamiliar with the techniques used in the facility, training sessions with full-time lab faculty, one-on-one instruction and assistance is also available when needed.
Histology Facility
The histology facility specializes in techniques that permit the visualization of tissue components at the resolution of the light microscope. A strong emphasis is placed on sample preparation for neuroanatomical and immunocytochemical studies. Microtomes are available to cut frozen,paraffin, vibratome, and plastic sections. Collaborative efforts, instruction, and technical support are included in the functions of the facility.
Monoclonal Antibody Facility
The Monoclonal Antibody Facility provides university researchers the opportunity to utilize current monoclonal antibody technology. As a result, the development and application of monoclonal antibodies has proven to be a popular, cost effective, and successful option for a variety of research groups. These collaborations have produced antibodies used to make advances in studies of molecular structure, subcellular activities, and cellular differentiation.
Facility services encompass every aspect of monoclonal antibody development, including generation of novel antibodies, large-scale antibody production and purification, and analysis of antibody binding specificities.
Genomics & Proteomics
The Genomics and Proteomics Lab has two capillary HPLCs, an LCQ Deca XP Plus ion trap tandem mass spectrometer equipped with both electrospray and AP Maldi sources, and an LTQ-FT hybrid linear ion trap-fourier transform ion cyclotron resonance mass spectrometer, allowing identification of proteins in complex mixtures by shotgun peptide sequencing. The lab also has various software tools for analysis of complex proteomics datasets, and additional analytical HPLCs. Other instrumentation includes robotics for sample manipulation, a Gene Axon 4000B DNA microarray slide scanner, a custom microarray printer, two tetrad PCRs, a real-time PCR instrument, and an Applied Biosystems 7900HT Sequence Detection System.
Bio-Optics Lab
The Bio-Optics lab is located in room 86, Lokey Laboratories, Integrative Science Complex.
Equipment available to users of the facility includes:
- a Bio-Rad Radiance 2100 confocal / two-photon microscope,
Zeiss Axioplan fluorescence microscope,
Zeiss universal microscope,
Leica stereo microscopes, equipped with digital cameras and video, and- Zeiss Stereo Discovery V.20 microscope, with Axiocam camera and Axiovision 4.7 imaging software.
Computer workstations are provided for processing digital images from the confocal and other lightmicroscopes. Software includes Improvision Volocity for 3D and 4D reconstruction, visualization, measurement, and analysis of confocal images.
Complete training and technical support is provided for all instruments and software. Other lab resources include large format poster printing and laminating, high quality digital photoprinting, film flatbed scanners, and copy stand. Technical support is always available for poster preparation and printing, and help is available for special photographic setups and photomicrography.
DNA Sequencing
The DNA Sequence lab provides sequencing service using two capillary-based Beckman Coulter CEQ DNA Analysis Systems. We use Dye Terminator Cycle sequencing chemistry and routinely provide over 700 base pairs per reaction on high quality template. The facility also houses two LiCor DNA Sequencers used primarily for high throughput genotyping.
Transgenic Mouse Facility
Since its opening in 2004, the Transgenic Mouse Facility has produced more than 6000 mice, representing 8 re-derived lines and 113 new lines. The TMF goal is to be able to provide all the services necessary to produce and maintain genetically modified mice. These services range from designing projects to maintaining colonies.
Integrative Science Complex
Providing state-of-the-art instruments that are fully staffed, professionally maintained and with maximum access for researchers. Science students are welcome to utilize the laboratories and the instrumentation under the direction of the CAMCOR staff. Training for all procedures and instrument operation is provided by the staff through regular academic courses, workshops and tutorials. The complex houses:
- Microanalytical Facility with EPMA and SEM
Surface Analytical Facility wth TOF-SIMS, Auger, XPS and AFM
Nanofabrication Facility with TEM, FIB and SEM e-Beamwriter
X-ray Crystallography Faciltiy with XRD
In addition, the Department of Biology also maintains:
• Two large greenhouses
• A large, up-to-date science library, located within the Science Complex
• Computerized biomedical literature searching facilities
• Two VAX computers
• A large animal care facility
• Considerably advanced optical microscopy
• A neuropsychological electronics shop
• An assortment of centrifuges and scintillation countersA complete science shop where electronic, mechanical, and glass devices can be built by professional staff members. Students and staff can fabricate their own research equipment in a special student shop.
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